Cytogenetic analysis of CpG-oligonucleotide DSP30 plus interleukin-2-stimulated canine B-cell lymphoma cells reveals the loss of one X chromosome as the sole abnormality

Reimann-Berg, N.; Murua Escobar, Hugo; Kiefer, Y.; Mischke, Reinhard; Willenbrock, S.; Eberle, Nina; Nolte, Ingo; Bullerdiek, J.

doi:10.1159/000330126

Published

Cytogenetic analysis of CpG-oligonucleotide DSP30 plus interleukin-2-stimulated canine B-cell lymphoma cells reveals the loss of one X chromosome as the sole abnormality

Reimann-Berg, N.; Murua Escobar, Hugo ; Kiefer, Y.; Mischke, Reinhard ; Willenbrock, S.; Eberle, Nina ; Nolte, Ingo ; Bullerdiek, J.

Human and canine lymphoid neoplasms are characterized by non-random cytogenetic abnormalities. However, due to the low mitotic activity of the B cells, cytogenetic analyses of B-cell lymphoid proliferations are difficult to perform. In the present study we stimulated canine B-cell lymphoma cells with the immunostimulatory CpG-oligonucleotide DSP30 in combination with interleukin-2 (IL-2) and obtained an adequate number of metaphases. Cytogenetic analyses revealed the loss of one X chromosome as the sole cytogenetic aberration. Chromosome analysis of the corresponding blood showed a normal female karyotype. Monosomy X as the sole clonal chromosomal abnormality is found in human hematopoietic malignancies as well, thus the dog may serve as a promising animal model.