Stiftung Tierärztliche Hochschule Hannover (TiHo)TiHo eLib

The effect of oxytocin stimulation on canine myometrial contractions in the organ bath

Oxytocin is the most common medication given in case of dystocia. In the dog, response to oxytocin in case of uterine inertia is usually poor, indicating the need for a caesarean section. Uterine interpla-cental tissue samples of 19 bitches were microscopically dissected, separating circular (CML) and longitudinal (LML) myometrial layers to analyse myometrial contractions in the organ bath. After equi-libration, strips of each layer were stimulated with the same oxy-tocin concentration (1 nM, 10 nM and 100 nM) twice with a 20-min washout in between. One untreated CML/LML strip each served as a control. Contractions were recorded and evaluated for average amplitude (A), mean force (MF) and frequency (F). Results were sta-tistically analysed for each stimulation separately using GraphPad Prism. Effects of different oxytocin concentrations were analysed and compared within layers as well as between both layers (CML/LML). During the first stimulation, unlike LML, all oxytocin concen-trations caused a significant increase in A, MF and F (all p< .05) in CML compared to the control. Whereas no significant differences between oxytocin and controls were identified during the second stimulation, 10 nM and 100 nM caused significantly lower A, MF and F (all p < .05) in LML compared to 1 nM. Comparing the response of the layers, 100 nM and 10 nM caused a significantly reduced F (all p < .01) and significantly decreased A, MF and F (all p < .001) in LML during the first and second stimulation, respectively. In summary, data indicate differences in functionality and oxytocin response of both myometrial layers.


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