Stiftung Tierärztliche Hochschule Hannover (TiHo)TiHo eLib

Ischaemic postconditioning - a potential therapeutic strategy for small intestinal ischaemia reperfusion injury in horses?

Background: Ischaemic postconditioning (IPoC) has been shown to ameliorate ischaemia reperfusion injury in different species and tissues. Objectives: To evaluate the feasibility of IPoC in equine small intestinal ischaemia and to assess its effect on histomorphology, electrophysiology and paracellular permeability. Methods: In this randomised controlled trial, experimental jejunal ischaemia was induced for 90 min in horses under general anaesthesia. In the control group (n = 7), the jejunum was reperfused without further intervention. In the postconditioning group (n = 7), reocclusion was implemented following release of ischaemia by clamping the mesenteric vessels with haemostatic forceps for 3 cycles of 30 sec. This was followed by 120 min of reperfusion in both groups. Intestinal microperfusion and oxygenation were measured using spectrophotometry and laser Doppler flowmetry. Mucosal histomorphology and histomorphometry as well as electrophysiological variables and 3 H-mannitol fluxrates were assessed. The expression of tight junction proteins claudin-1, claudin-2 and occludin was determined by Western blot analysis. Results: IPoC significantly reduced intestinal microperfusion during all clamping cycles, yet affected tissue oxygen saturation only during the first cycle. After reperfusion, group IPoC showed significantly less epithelial villus denudation (mean difference 21.5%, p = 0.02) and decreased mucosalto-serosal fluxrates (mean difference 15.2 nM/cm2 /h, p = 0.007) compared to the control group. There were no significant differences between the groups for the other tested variables. Conclusions: Following IPoC, the intestinal mucosa demonstrated significantly reduced epithelial denudation and paracellular permeability compared to the untreated control group, possibly indicating a protective effect of IPoC on ischaemia reperfusion injury.

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