Stiftung Tierärztliche Hochschule Hannover (TiHo)

Simple targeted assays for metabolic pathways and signaling : a powerful tool for targeted proteomics

Kopczynski, Dominik ORCID; Hentschel, Andreas; Coman, Cristina ORCID; Schebb, Nils Helge GND; Hornemann, Thorsten; Mashek, Douglas G; Hartung, Nicole M; Shevchuk, Olga; Schött, Hans-Frieder; Lorenz, Kristina; Torta, Federico; Burla, Bo ORCID; Zahedi, René P; Sickmann, Albert; Kreutz, Michael R; Ejsing, Christer S ORCID; Medenbach, Jan; Ahrends, Robert ORCID

We introduce STAMPS, a pathway-centric web service for the development of targeted proteomics assays. STAMPS guides the user by providing several intuitive interfaces for a rapid and simplified method design. Applying our curated framework to signaling and metabolic pathways, we reduced the average assay development time by a factor of ∼150 and revealed that the insulin signaling is actively controlled by protein abundance changes in insulin-sensitive and -resistance states. Although at the current state STAMPS primarily contains mouse data, it was designed for easy extension with additional organisms.

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