Stiftung Tierärztliche Hochschule Hannover (TiHo)

Curcumin mitigates immune-induced epithelial barrier dysfunction by Campylobacter jejuni

Lobo de Sá, Fábia Daniela ORCID; Butkevych, Eduard; Nattramilarasu, Praveen Kumar; Fromm, Anja ORCID; Mousavi, Soraya; Moos, Verena ORCID; Golz, Julia C.; Stingl, Kerstin; Kittler, Sophie ORCID; Seinige, Diana; Kehrenberg, Corinna; Heimesaat, Markus M.; Bereswill, Stefan; Schulzke, Jörg-Dieter ORCID; Bücker, Roland ORCID

Campylobacter jejuni (C. jejuni) is the most common cause of foodborne gastroenteritis worldwide. The bacteria induce diarrhea and inflammation by invading the intestinal epithelium. Curcumin is a natural polyphenol from turmeric rhizome of Curcuma longa, a medical plant, and is commonly used in curry powder. The aim of this study was the investigation of the protective effects of curcumin against immune-induced epithelial barrier dysfunction in C. jejuni infection. The indirect C. jejuni-induced barrier defects and its protection by curcumin were analyzed in co-cultures with HT-29/B6-GR/MR epithelial cells together with differentiated THP-1 immune cells. Electrophysiological measurements revealed a reduction in transepithelial electrical resistance (TER) in infected co-cultures. An increase in fluorescein (332 Da) permeability in co-cultures as well as in the germ-free IL-10-/- mouse model after C. jejuni infection was shown. Curcumin treatment attenuated the C. jejuni-induced increase in fluorescein permeability in both models. Moreover, apoptosis induction, tight junction redistribution, and an increased inflammatory response-represented by TNF-α, IL-1β, and IL-6 secretion-was observed in co-cultures after infection and reversed by curcumin. In conclusion, curcumin protects against indirect C. jejuni-triggered immune-induced barrier defects and might be a therapeutic and protective agent in patients.

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Lobo de Sá, F.D., Butkevych, E., Nattramilarasu, P.K., Fromm, A., Mousavi, S., Moos, V., Golz, J.C., Stingl, K., Kittler, S., Seinige, D., Kehrenberg, C., Heimesaat, M.M., Bereswill, S., Schulzke, J.-D., Bücker, R., 2019. Curcumin mitigates immune-induced epithelial barrier dysfunction by Campylobacter jejuni. International journal of molecular sciences 20. https://doi.org/10.3390/ijms20194830
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