Stiftung Tierärztliche Hochschule Hannover (TiHo)

Cows selected for divergent mastitis susceptibility display a differential liver transcriptome profile after experimental Staphylococcus aureus mammary gland inoculation

ORCID
0000-0003-0638-0596
Affiliation
Leibniz Institute for Farm Animal Biology (FBN), Institute of Genome Biology, 18196 Dummerstorf, Germany.
Heimes, A.;
Affiliation
Leibniz Institute for Farm Animal Biology (FBN), Institute of Genome Biology, 18196 Dummerstorf, Germany.
Brodhagen, J.;
Affiliation
Leibniz Institute for Farm Animal Biology (FBN), Institute of Genome Biology, 18196 Dummerstorf, Germany.
Weikard, R.;
ORCID
0000-0003-0425-6284
Affiliation
Leibniz Institute for Farm Animal Biology (FBN), Institute of Genome Biology, 18196 Dummerstorf, Germany.
Becker, D.;
GND
105362641X
ORCID
0000-0003-1720-6593
Affiliation
Clinic for Ruminants with Ambulatory and Herd Health Services, Centre for Clinical Veterinary Medicine, Ludwig-Maximilians-University Munich, 85764 Oberschleißheim, Germany; Immunology Unit, University of Veterinary Medicine Hannover, 30559 Hannover, Germany.
Meyerholz, Marie Margarete;
ORCID
0000-0002-7931-2642
Affiliation
Clinic for Ruminants with Ambulatory and Herd Health Services, Centre for Clinical Veterinary Medicine, Ludwig-Maximilians-University Munich, 85764 Oberschleißheim, Germany.
Petzl, W.;
ORCID
0000-0002-7972-4858
Affiliation
Clinic for Ruminants with Ambulatory and Herd Health Services, Centre for Clinical Veterinary Medicine, Ludwig-Maximilians-University Munich, 85764 Oberschleißheim, Germany.
Zerbe, H.;
ORCID
0000-0002-5904-5751
Affiliation
Immunology Unit, University of Veterinary Medicine Hannover, 30559 Hannover, Germany.
Schuberth, Hans-Joachim;
GND
118032224
ORCID
0000-0001-8154-2642
Affiliation
Clinic for Cattle, University of Veterinary Medicine Hannover, 30173 Hannover, Germany.
Hoedemaker, Martina;
GND
133948544
ORCID
0000-0001-7218-293X
Affiliation
Faculty of Natural Sciences III, Martin-Luther Universität Halle-Wittenberg, 06120 Halle, Germany.
Schmicke, Marion;
Affiliation
Technical University Braunschweig, Institute for Microbiology, 38023 Braunschweig, Germany; Helmholtz Centre for Infection Research, Microbial Proteomics, 38124 Braunschweig, Germany.
Engelmann, S.;
Affiliation
Leibniz Institute for Farm Animal Biology (FBN), Institute of Genome Biology, 18196 Dummerstorf, Germany; Agricultural and Environmental Faculty, University Rostock, 18059 Rostock, Germany. Electronic address: kuehn@fbn-dummerstorf.de.
Kühn, C.

Infection and inflammation of the mammary gland, and especially prevention of mastitis, are still major challenges for the dairy industry. Different approaches have been tried to reduce the incidence of mastitis. Genetic selection of cows with lower susceptibility to mastitis promises sustainable success in this regard. Bos taurus autosome (BTA) 18, particularly the region between 43 and 59 Mb, harbors quantitative trait loci (QTL) for somatic cell score, a surrogate trait for mastitis susceptibility. Scrutinizing the molecular bases hereof, we challenged udders from half-sib heifers having inherited either favorable paternal haplotypes for somatic cell score (Q) or unfavorable haplotypes (q) with the Staphylococcus aureus pathogen. RNA sequencing was used for an in-depth analysis of challenge-related alterations in the hepatic transcriptome. Liver exerts highly relevant immune functions aside from being the key metabolic organ. Hence, a holistic approach focusing on the liver enabled us to identify challenge-related and genotype-dependent differentially expressed genes and underlying regulatory networks. In response to the S. aureus challenge, we found that heifers with Q haplotypes displayed more activated immune genes and pathways after S. aureus challenge compared with their q half-sibs. Furthermore, we found a significant enrichment of differentially expressed loci in the genomic target region on BTA18, suggesting the existence of a regionally acting regulatory element with effects on a variety of genes in this region.

Cite

Citation style:
Could not load citation form.

Rights

Use and reproduction:

Export