Stiftung Tierärztliche Hochschule Hannover (TiHo)

A comparison of different vaccination schemes used in sheep combining inactivated bluetongue vaccines against serotypes 4 and 8

Affiliation
Sheep Veterinary Practice Strobel, Am Hopfenberg 8, 89352 Stoffenried, Germany; Clinic for Swine and Small Ruminants, Forensic Medicine and Ambulatory Service, University of Veterinary Medicine Hannover, Foundation, Bischofsholer Damm 15, 30173 Hannover, Germany. Electronic address: johanna.hilke@tiho-hannover.de.
Hilke, Johanna;
Affiliation
Sheep Veterinary Practice Strobel, Am Hopfenberg 8, 89352 Stoffenried, Germany.
Strobel, Heinz;
Affiliation
Friedrich-Loeffler-Institute, Federal Research Institute for Animal Health, Suedufer 10, 17493 Greifswald - Riems, Germany.
Woelke, Soeren;
Affiliation
Clinic for Swine and Small Ruminants, Forensic Medicine and Ambulatory Service, University of Veterinary Medicine Hannover, Foundation, Bischofsholer Damm 15, 30173 Hannover, Germany.
Stoeter, Melanie;
Affiliation
Clinic for Ruminants with Ambulatory and Herd Health Services, Ludwig-Maximilians-University Munich, Sonnenstr. 16, 85764 Oberschleissheim, Germany.
Voigt, Katja;
Affiliation
Clinic for Swine and Small Ruminants, Forensic Medicine and Ambulatory Service, University of Veterinary Medicine Hannover, Foundation, Bischofsholer Damm 15, 30173 Hannover, Germany.
Grimm, Lucie;
Affiliation
Clinic for Swine and Small Ruminants, Forensic Medicine and Ambulatory Service, University of Veterinary Medicine Hannover, Foundation, Bischofsholer Damm 15, 30173 Hannover, Germany.
Meilwes, Johanna;
ORCID
0000-0003-0140-6339
Affiliation
Clinic for Swine and Small Ruminants, Forensic Medicine and Ambulatory Service, University of Veterinary Medicine Hannover, Foundation, Bischofsholer Damm 15, 30173 Hannover, Germany.
Punsmann, Teresa;
Affiliation
State Veterinary Investigation Centre Aulendorf, Loewenbreitestr. 20, 88326 Aulendorf, Germany.
Blaha, Irena;
Affiliation
State Veterinary Investigation Centre Aulendorf, Loewenbreitestr. 20, 88326 Aulendorf, Germany.
Salditt, Andreas;
GND
136929192
Affiliation
Institute for Biometry, Epidemiology and Information Processing, University of Veterinary Medicine, Hannover, Foundation, Buenteweg 2, 30559 Hannover, Germany.
Rohn, Karl;
Affiliation
Friedrich-Loeffler-Institute, Federal Research Institute for Animal Health, Suedufer 10, 17493 Greifswald - Riems, Germany.
Bastian, Max;
GND
1050572742
ORCID
0000-0003-1589-0803
Affiliation
Clinic for Swine and Small Ruminants, Forensic Medicine and Ambulatory Service, University of Veterinary Medicine Hannover, Foundation, Bischofsholer Damm 15, 30173 Hannover, Germany.
Ganter, Martin

Eight different vaccination schemes using four commercially available inactivated Bluetongue vaccines against serotypes 4 and 8 in three different combinations (setting 1-3) were tested under field conditions for their ability to generate a measurable immune response in sheep. Animals of setting 1 (groups A-D) were simultaneously vaccinated using either individual injections at different locations (groups A & D) or double injection by a twin-syringe (groups B & C). For both application methods, a one-shot vaccination (groups C & D) was compared to a boosted vaccination (groups A & B). Sheep of setting 2 (groups E-G) were vaccinated in an alternating, boosted pattern at fortnightly intervals starting with serotype 4 (groups E & F) or vice versa (group G). Group H of setting 3 was vaccinated simultaneously and vaccines were injected individually as a one-shot application. Each group consisted of 30 sheep. The immunogenic response was tested in all sheep (n = 240) by ELISA (IDScreen®Bluetongue Competition), while serum neutralisation tests were performed in five to six sheep from each group (n = 45). All vaccine combinations were well tolerated by all sheep. Of all vaccines and schemes described, the simultaneous double injected boosted vaccination of setting 1 (group B) yielded the highest median serotype-specific titres 26 weeks after the first vaccination (afv) and 100% seropositive animals (ELISA) one year afv. In setting 1, there were no relevant significant differences in the immunogenic response between simultaneously applied vaccines at different sites or at the same injection site. Importantly, a one-shot vaccination induced comparable immunogenicity to a boosted injection half a year afv. Low serotype-specific neutralising antibody levels were detected in settings 2 and 3 and are attributed to diverse factors which may have influenced the measured immunogenicity.

Cite

Citation style:
Could not load citation form.

Rights

Use and reproduction:
All rights reserved

Export