Stiftung Tierärztliche Hochschule Hannover (TiHo)

Influence of Oxygen on Function and Cholesterol Composition of Murine Bone Marrow-Derived Neutrophils.

GND
1043076840
Affiliation
Department of Physiological Chemistry, University of Veterinary Medicine Hannover, Hannover, Germany.
Branitzki-Heinemann, Katja;
GND
1047165775
Affiliation
Department of Physiological Chemistry, University of Veterinary Medicine Hannover, Hannover, Germany.
Brogden, Graham;
GND
129745138
ORCID
0000-0001-7712-9767
Affiliation
Department of Physiological Chemistry, University of Veterinary Medicine Hannover, Hannover, Germany. maren.von.koeckritz-blickwede@tiho-hannover.de.
von Köckritz-Blickwede, Maren

During inflammation and infection, invading pathogens as well as infiltrating neutrophils locally consume oxygen and reduce the present oxygen level. Since oxygen is an elementary component of the microenvironment required for cell activity and alters multiple cellular functions, it is important to study neutrophil functionality and phenotype at characteristic pathophysiological oxygen levels that reflect the hypoxic phenotype during infection and inflammation. Here, we describe methods to study murine neutrophils under hypoxic compared to normoxic conditions, including analysis of cholesterol content as a key lipid involved in biological functions.

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