Stiftung Tierärztliche Hochschule Hannover (TiHo)

A sensitive scoring system for the longitudinal clinical evaluation and prediction of lethal disease outcomes in newborn mice

Fehlhaber, Beate; Heinemann, Anna S; Rübensam, Kathrin GND; Willers, Maike; Völlger, Lena; Pfeifer, Sandra GND; von Köckritz-Blickwede, Maren GND; Viemann, Dorothee

Neonatal animal models are increasingly employed in order to unravel age-specific disease mechanisms. Appropriate tools objectifying the clinical condition of murine neonates are lacking. In this study, we tested a scoring system specifically designed for newborn mice that relies on clinical observation and examination. Both, in a neonatal sepsis model and an endotoxic shock model, the scoring results strongly correlated with disease-induced death rates. Full as well as observation-restricted scoring, reliably predicted fatality and the remaining time until death. Clinical scores even proved as more sensitive biomarker than 6 traditionally used plasma cytokine levels in detecting sepsis at an early disease stage. In conclusion, we propose a simple scoring system that detects health impairments of newborn mice in a non-invasive longitudinal and highly sensitive manner. Its usage will help to meet animal welfare requirements and might improve the understanding of neonatal disease mechanisms.


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