Stiftung Tierärztliche Hochschule Hannover (TiHo)

A sensitive scoring system for the longitudinal clinical evaluation and prediction of lethal disease outcomes in newborn mice

Affiliation
Department of Paediatric Pneumology, Allergology and Neonatology, Hannover Medical School, 30625, Hannover, Germany.
Fehlhaber, Beate;
Affiliation
Department of Paediatric Pneumology, Allergology and Neonatology, Hannover Medical School, 30625, Hannover, Germany.
Heinemann, Anna S;
GND
1074532961
Affiliation
Research Centre for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, 30559, Hannover, Germany.
Rübensam, Kathrin;
Affiliation
Department of Paediatric Pneumology, Allergology and Neonatology, Hannover Medical School, 30625, Hannover, Germany.
Willers, Maike;
Affiliation
Department of Paediatric Pneumology, Allergology and Neonatology, Hannover Medical School, 30625, Hannover, Germany.
Völlger, Lena;
Affiliation
Department of Physiological Chemistry, University of Veterinary Medicine Hannover, 30559, Hannover, Germany.
Pfeifer, Sandra;
GND
129745138
ORCID
0000-0001-7712-9767
Affiliation
Research Centre for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, 30559, Hannover, Germany.
von Köckritz-Blickwede, Maren;
Affiliation
Department of Paediatric Pneumology, Allergology and Neonatology, Hannover Medical School, 30625, Hannover, Germany. viemann.dorothee@mh-hannover.de.
Viemann, Dorothee

Neonatal animal models are increasingly employed in order to unravel age-specific disease mechanisms. Appropriate tools objectifying the clinical condition of murine neonates are lacking. In this study, we tested a scoring system specifically designed for newborn mice that relies on clinical observation and examination. Both, in a neonatal sepsis model and an endotoxic shock model, the scoring results strongly correlated with disease-induced death rates. Full as well as observation-restricted scoring, reliably predicted fatality and the remaining time until death. Clinical scores even proved as more sensitive biomarker than 6 traditionally used plasma cytokine levels in detecting sepsis at an early disease stage. In conclusion, we propose a simple scoring system that detects health impairments of newborn mice in a non-invasive longitudinal and highly sensitive manner. Its usage will help to meet animal welfare requirements and might improve the understanding of neonatal disease mechanisms.

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