Stiftung Tierärztliche Hochschule Hannover (TiHo)

Suitability of ultrasound-guided fine-needle aspiration biopsy for transcriptome sequencing of the canine prostate

ORCID
0000-0002-0136-2711
Affiliation
Small Animal Clinic, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany.
Thiemeyer, Heike;
Affiliation
Division of Bioinformatics, Department of Biology, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany.
Taher, L.;
ORCID
0000-0001-9736-7105
Affiliation
Small Animal Clinic, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany.
Schille, Jan Torben;
Affiliation
Small Animal Clinic, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany.
Harder, Lisa;
GND
131840703
ORCID
0000-0001-9934-3879
Affiliation
Small Animal Clinic, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany.
Hungerbühler, Stefan;
GND
130569054
Affiliation
Small Animal Clinic, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany.
Mischke, Reinhard;
ORCID
0000-0002-4278-331X
Affiliation
Institute of Pathology, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany.
Hewicker-Trautwein, Marion;
Affiliation
Department and Clinic of Veterinary Surgery, Faculty of Veterinary Medicine, Wroclaw University of Environmental and Life Sciences, Wrocław, Poland.
Kiełbowicz, Z.;
Affiliation
University of Göttingen, Institute of Veterinary Medicine, Göttingen, Germany.
Brenig, B.;
Affiliation
Chronix Biomedical, Göttingen, Germany.
Schütz, E.;
Affiliation
Chronix Biomedical, Göttingen, Germany.
Beck, J.;
GND
12980455X
ORCID
0000-0001-7123-7986
Affiliation
Small Animal Clinic, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany.
Murua Escobar, Hugo;
GND
1046299905
ORCID
0000-0003-4577-3739
Affiliation
Small Animal Clinic, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany. Ingo.Nolte@tiho-hannover.de.
Nolte, Ingo

Ultrasound-guided fine-needle aspiration (US-FNA) biopsy is a widely used minimally invasive sampling procedure for cytological diagnosis. This study investigates the feasibility of using US-FNA samples for both cytological diagnosis and whole transcriptome RNA-sequencing analysis (RNA-Seq), with the ultimate aim of improving canine prostate cancer management. The feasibility of the US-FNA procedure was evaluated intra vitam on 43 dogs. Additionally, aspirates from 31 euthanised dogs were collected for standardising the procedure. Each aspirate was separated into two subsamples: for cytology and RNA extraction. Additional prostate tissue samples served as control for RNA quantity and quality evaluation, and differential expression analysis. The US-FNA sampling procedure was feasible in 95% of dogs. RNA isolation of US-FNA samples was successfully performed using phenol-chloroform extraction. The extracted RNA of 56% of a subset of US-FNA samples met the quality requirements for RNA-Seq. Expression analysis revealed that only 153 genes were exclusively differentially expressed between non-malignant US-FNAs and tissues. Moreover, only 36 differentially expressed genes were associated with the US-FNA sampling technique and unrelated to the diagnosis. Furthermore, the gene expression profiles clearly distinguished between non-malignant and malignant samples. This proves US-FNA to be useful for molecular profiling.

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