Stiftung Tierärztliche Hochschule Hannover (TiHo)

Detection of MERS-CoV antigen on formalin-fixed paraffin-embedded nasal tissue of alpacas by immunohistochemistry using human monoclonal antibodies directed against different epitopes of the spike protein

Affiliation
Department of Pathology, University of Veterinary Medicine Hannover Foundation, 30559 Hannover, Germany.
Haverkamp, Ann-Kathrin;
ORCID
0000-0002-3864-232X
Affiliation
Virology Division, Department of Infectious Diseases & Immunology, Faculty of Veterinary Medicine, Utrecht University, 3584 CL Utrecht, the Netherlands.
Bosch, Berend-Jan;
ORCID
0000-0001-9935-3700
Affiliation
Department of Pathology, University of Veterinary Medicine Hannover Foundation, 30559 Hannover, Germany; Center for Systems Neuroscience, 30559 Hannover, Germany.
Spitzbarth, Ingo;
GND
1023386887
ORCID
0000-0002-6970-4327
Affiliation
Department of Pathology, University of Veterinary Medicine Hannover Foundation, 30559 Hannover, Germany; Center for Systems Neuroscience, 30559 Hannover, Germany.
Lehmbecker, Annika;
Affiliation
IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.
Te, Nigeer;
Affiliation
IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.
Bensaid, Albert;
Affiliation
Departament de Sanitat i Anatomia Animals, Facultat de Veterinària, UAB, 08193 Bellaterra, Barcelona, Spain; UAB, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.
Segalés, Joaquim;
GND
142929565
ORCID
0000-0001-8151-5644
Affiliation
Department of Pathology, University of Veterinary Medicine Hannover Foundation, 30559 Hannover, Germany; Center for Systems Neuroscience, 30559 Hannover, Germany. Electronic address: Wolfgang.Baumgaertner@tiho-hannover.de.
Baumgärtner, Wolfgang

Middle East respiratory syndrome (MERS) represents an important respiratory disease accompanied by lethal outcome in one third of human patients. In recent years, several investigators developed protective antibodies which could be used as prophylaxis in prospective human epidemics. In the current study, eight human monoclonal antibodies (mAbs) with neutralizing and non-neutralizing capabilities, directed against different epitopes of the MERS-coronavirus (MERS-CoV) spike (MERS-S) protein, were investigated with regard to their ability to immunohistochemically detect respective epitopes on formalin-fixed paraffin-embedded (FFPE) nasal tissue sections of MERS-CoV experimentally infected alpacas. The most intense immunoreaction was detected using a neutralizing antibody directed against the receptor binding domain S1B of the MERS-S protein, which produced an immunosignal in the cytoplasm of ciliated respiratory epithelium and along the apical membranous region. A similar staining was obtained by two other mAbs which recognize the sialic acid-binding domain and the ectodomain of the membrane fusion subunit S2, respectively. Five mAbs lacked immunoreactivity for MERS-CoV antigen on FFPE tissue, even though they belong, at least in part, to the same epitope group. In summary, three tested human mAbs demonstrated capacity for detection of MERS-CoV antigen on FFPE samples and may be implemented in double or triple immunohistochemical methods.

Cite

Citation style:
Could not load citation form.

Rights

Use and reproduction:
All rights reserved

Export