Stiftung Tierärztliche Hochschule Hannover (TiHo)

The CARD9-associated C-type lectin, mincle, recognizes La Crosse virus (LACV) but plays a limited role in early antiviral responses against LACV

ORCID
0000-0002-1549-8855
Affiliation
Immunology Unit & Research Center for Emerging Infections and Zoonoses, University of Veterinary Medicine Hannover, 30559 Hannover, Germany. joao.monteiro@tiho-hannover.de.
Monteiro, João;
Affiliation
Immunology Unit & Research Center for Emerging Infections and Zoonoses, University of Veterinary Medicine Hannover, 30559 Hannover, Germany. kathleen.schoen@tiho-hannover.de.
Schön, Kathleen;
Affiliation
Immunology Unit & Research Center for Emerging Infections and Zoonoses, University of Veterinary Medicine Hannover, 30559 Hannover, Germany. tim.markus.ebbecke@tiho-hannover.de.
Ebbecke, Tim;
GND
1079298096
ORCID
0000-0002-0348-3090
Affiliation
Institute for Microbiology, University of Veterinary Medicine Hannover, 30173 Hannover, Germany. ralph.goethe@tiho-hannover.de.
Goethe, Ralph;
Affiliation
Institute of Clinical Chemistry and Pathobiochemistry, School of Medicine, Technical University of Munich, 81675 Munich, Germany. j.ruland@tum.de.
Ruland, Jürgen;
GND
142929565
ORCID
0000-0001-8151-5644
Affiliation
Department of Pathology, University of Veterinary Medicine Hannover, 30559 Hannover, Germany. wolfgang.baumgaertner@tiho-hannover.de.
Baumgärtner, Wolfgang;
GND
133156192
Affiliation
Institute for Parasitology and & Research Center for Emerging Infections and Zoonoses, University of Veterinary Medicine Hannover, 30559 Hannover, Germany. stefanie.becker@tiho-hannover.de.
Becker, Stefanie;
GND
133829545
ORCID
0000-0001-9033-5522
Affiliation
Immunology Unit & Research Center for Emerging Infections and Zoonoses, University of Veterinary Medicine Hannover, 30559 Hannover, Germany. bernd.lepenies@tiho-hannover.de.
Lepenies, Bernd

La Crosse virus (LACV) is a mosquito-transmitted arbovirus and the main cause of virus-mediated neurological diseases in children. To date, little is known about the role of C-type lectin receptors (CLRs)-an important class of pattern recognition receptors-in LACV recognition. DC-SIGN remains the only well-described CLR that recognizes LACV. In this study, we investigated the role of additional CLR/LACV interactions. To this end, we applied a flow-through chromatography method for the purification of LACV to perform an unbiased high-throughput screening of LACV with a CLR-hFc fusion protein library. Interestingly, the CARD9-associated CLRs Mincle, Dectin-1, and Dectin-2 were identified to strongly interact with LACV. Since CARD9 is a common adaptor protein for signaling via Mincle, Dectin-1, and Dectin-2, we performed LACV infection of Mincle-/- and CARD9-/- DCs. Mincle-/- and CARD9-/- DCs produced less amounts of proinflammatory cytokines, namely IL-6 and TNF-α, albeit no reduction of the LACV titer was observed. Together, novel CLR/LACV interactions were identified; however, the Mincle/CARD9 axis plays a limited role in early antiviral responses against LACV.

Cite

Citation style:
Could not load citation form.

Rights

Use and reproduction:

Export