Stiftung Tierärztliche Hochschule Hannover (TiHo)

Microbe-focused glycan array screening platform

Affiliation
Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany.
Geissner, Andreas;
Affiliation
Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany.
Reinhardt, Anika;
ORCID
0000-0001-7082-7239
Affiliation
Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany.
Rademacher, Christoph;
Affiliation
Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany.
Johannssen, Timo;
ORCID
0000-0002-9855-7485
Affiliation
Immunology Unit and Research Center for Emerging Infections and Zoonoses, University of Veterinary Medicine Hannover, 30559 Hannover, Germany.
Monteiro, João;
GND
133829545
ORCID
0000-0001-9033-5522
Affiliation
Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany.
Lepenies, Bernd;
Affiliation
Université Grenoble Alpes, Institut de Biologie Structurale, F-38044 Grenoble, France.
Thépaut, Michel;
ORCID
0000-0003-1194-8107
Affiliation
Université Grenoble Alpes, Institut de Biologie Structurale, F-38044 Grenoble, France.
Fieschi, Franck;
Affiliation
Central European Institute of Technology, Masaryk University, 625 00 Brno, Czech Republic.
Mrázková, Jana;
ORCID
0000-0002-7108-4198
Affiliation
Central European Institute of Technology, Masaryk University, 625 00 Brno, Czech Republic.
Wimmerova, Michaela;
ORCID
0000-0002-2586-4118
Affiliation
Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany.
Schuhmacher, Frank;
ORCID
0000-0002-4087-0225
Affiliation
Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany.
Götze, Sebastian;
Affiliation
Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany.
Grünstein, Dan;
Affiliation
Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany.
Guo, Xiaoqiang;
ORCID
0000-0003-2326-7923
Affiliation
Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany.
Hahm, Heung Sik;
ORCID
0000-0003-3285-971X
Affiliation
Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany.
Kandasamy, Jeyakumar;
Affiliation
Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany.
Leonori, Daniele;
Affiliation
Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany.
Martin, Christopher E.;
Affiliation
Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany.
Parameswarappa, Sharavathi G.;
Affiliation
Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany.
Pasari, Sandip;
Affiliation
Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany.
Schlegel, Mark K.;
Affiliation
Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany.
Tanaka, Hidenori;
Affiliation
Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany.
Xiao, Guozhi;
ORCID
0000-0003-4438-2162
Affiliation
Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany.
Yang, You;
Affiliation
Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany.
Pereira, Claney L.;
Affiliation
Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany.
Anish, Chakkumkal;
ORCID
0000-0003-3394-8466
Affiliation
Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, 14424 Potsdam, Germany; peter.seeberger@mpikg.mpg.de.
Seeberger, Peter H.

Interactions between glycans and glycan binding proteins are essential for numerous processes in all kingdoms of life. Glycan microarrays are an excellent tool to examine protein-glycan interactions. Here, we present a microbe-focused glycan microarray platform based on oligosaccharides obtained by chemical synthesis. Glycans were generated by combining different carbohydrate synthesis approaches including automated glycan assembly, solution-phase synthesis, and chemoenzymatic methods. The current library of more than 300 glycans is as diverse as the mammalian glycan array from the Consortium for Functional Glycomics and, due to its microbial focus, highly complementary. This glycan platform is essential for the characterization of various classes of glycan binding proteins. Applications of this glycan array platform are highlighted by the characterization of innate immune receptors and bacterial virulence factors as well as the analysis of human humoral immunity to pathogenic glycans.

Cite

Citation style:
Could not load citation form.

Rights

Use and reproduction:
All rights reserved

Export